BCM 230 Lectures

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Lecture #1: Nuclear spins & energy levels, allowed transitions, population of spin states, precession of vectors, Larmor frequency, basic NMR observables, correlation of shift and integrals to structure. Pulsed FT experiment, comparison of classical & quantum mechanical pictures of NMR experiment, pulse widths, observation of NMR signals, components of NMR spectrometer.

Lecture #2. Rotating frame, rotation of magnetization, recovery of magnetization, carrier frequency, interpretation of free induction decay, Nyquist theorem and sampling of the FID, quadrature detection, survey of the Fourier transform. Real and imaginary solutions of the FT, phase corrections(zero and first order), apodization of the FID.

Lecture #3. Probe design & tuning, deuterium lock, sample shimming, signal clipping, analog to digital conversion, repetition rates of pulse sequences, pulse calibration.

Lecture #4. Nuclear spin relaxation, difference between T1 and T2 , nuclear relaxation in the rotating frame, mechanisms of spin relaxation, connection of relaxation with correlation times of nuclear motions, measurement of spin-lattice relaxation, spin echoes, transverse relaxation, measurement of spin-spin relaxation, spectral editing.

Lecture #5. Mechanism of spin coupling, effect in spectra, n+1 rule, selective homonuclear decoupling & use for peak assignments, broadband heteronuclear decoupling. Mechanism of NOE, use of homonuclear NOE for assignments & internuclear distances, heteronuclear NOE. Solvent suppression.

Lecture #6. Two-dimensional NMR: evolution of frequencies in two dimensions, 2D FT, pulse sequences for 2D NMR, sampling rates of 2D signals, specific example of a 2D pulse sequence in the rotating frame.

Lecture #7. Two dimensional NMR: examples of spin and dipolar correlation experiments, utility for peak assignments and 3D structural determinations.

Lecture #8. MR imaging: field gradients, selective pulses, slice selection, frequency and phase encoding, spin-echo imaging, gradient echo imaging, fast imaging.

Lecture #9. Image contrast:  T1 and T2 weighted imaging, localized spectroscopy: surface coils, gradient localized methods, chemical shift imaging.

Lecture #10. NMR and MRI of diffusion, flow and solids.

Note: some lectures are expanded and others compressed or combined from year to year.