CURRICULUM VITAE
JEFFREY S. DE ROPP
NMR Facility
UC Davis
jsderopp@ucdavis.edu
B.S. Chemistry (Honors) 1976
Ph.D.
Physical Chemistry 1981 Univ. of
1976-77: Teaching Assistant,
Chemistry Dept., UC Davis
1977-81: Research Assistant,
Chemistry Dept., UC Davis
1981-84: Post-Doctoral
Researcher, Department of Biological Chemistry, UC Davis.
1985-present: Assistant,
Associate, Full Research Spectroscopist, and Manager, NMR Facility, UC Davis
RESEARCH
Study
of the structure and dynamics, and their correlation to biological function, of
high molecular weight heme proteins. This research has revealed the active site
structure essential to the catalytic function of heme peroxidases; the
methodology first developed in these studies has been successfully applied to
other heme proteins (Publications 1-12, 14, 16-19, 21, 25 listed below).
Additional work has focused on extending the frontiers of 2D NMR techniques to
include paramagnetic systems (29-32, 34), and the application of these methods
to structural analysis of large metalloenzymes
(36-40, 44, 48, 50). The cofactor binding site, and the thermodynamics and
kinetics of cofactor binding in horseradish peroxidase have also been determined
(33, 43). The work in this field has been summarized in two important reviews
(35, 45).
Collaborative
research projects at the NMR Facility have also included:
Development of a proton NMR method for forensic analysis (23).
A novel in vitro deuterium
NMR investigation of glycogen metabolism in the rat (26).
Study
of the molecular solution conformation of protein kinase
inhibitor peptides (27).
Magnetic resonance imaging studies of freezing of kiwifruit (41), and a review of magnetic resonance imaging and spectroscopic studies of plants and fruits (47).
Development of NMR as a tool for paleobiology
(42).
Study of the interaction of phenolics and
aromatic flavor compounds (46, 51).
Application of high field magnetic resonance imaging for the mouse
(49).
Collaborative analysis of the structure of marine natural products
(52).
Fabrication and testing of novel NMR microcoils
(53).
Post-doctoral
research:
Deuterium NMR investigations of polyisoprenol (PI)
molecular dynamics. These ubiquitous but poorly understood molecules function
as glycosyl carrier lipids in cell membranes.
Deuterium NMR investigation of PIs in model membranes provided the first
detailed evidence of the molecular conformations and motions of PIs to shed
light on their structure/function relationships (13, 15, 20).
Teaching
Biological
Chemistry 230:
NMR Spectroscopy & Imaging
Foundational lecture and laboratory course (offered annually) to train
graduate students and post-doctoral researchers from across campus in NMR
theory and application. Self-published a 120-page text for this class, as well as two detailed
manuals to instruct students in hands-on usage of the spectrometers. Have trained over 200 students to be users of Facility
instrumentation. Teaching evaluations for over-all effectiveness are
consistently >4.5 on a scale of 1-5.
Team-Taught
Courses have included:
Molecular
& Cellular Biology 221A: Biophysical Chemistry
Zoology
200: Topics
in Zoology
Biological
Chemistry 216:
Protein Structure
Biochemistry
and Biophysics 202B: Techniques in Biochemistry
Environmental
Toxicology 112B: Toxicants in the Environment
Environmental
Toxicology 220:
Analysis of Toxicants
Psychology
190/290:
Functional Magnetic Resonance Imaging
Campus
AND PUBLIC Service Activity
Regular Campus Committees
1. Academic Federation Committee on
Committees, 2001-2002, 2002-2003. Determine membership of all Academic
Federation committees and select AF officers. Chair of Committee for
2002-2003.
2. Academic Federation Administrative
Series Personnel Committee, 2002-2003, 2003-4. Review and recommend action on
appointments, merits, promotions, and position screenings for the personnel in
the Academic Federation administrative series titles.
3. Research
Sub-Committee of Academic Computing Coordinating Council (AC4),
2001-2002, 2002-3. Advise IT on research needs of campus, particularly support
of faculty research, and security issues.
4. Graduate Council, 2000-2001. Reviewed
graduate programs and made recommendations on improvement. Worked
on issues concerned with graduate student funding support. Member of Academic Planning and Development sub-committee that
drafted first ever comprehensive campus-wide policies for hiring, promotion,
and mentoring of post-doctoral scholars.
5. Academic Federation Executive Council,
1997-1998; 1998-1999; 2002-2003. Addressed issues pertaining to AF members,
including merit & promotion processes, gender equity, academic freedom,
educational and research policies.
6. OVCR Council of Principal
Investigators, 1997-98; 1998-1999. Participated in advising
the Vice-Chancellor for Research on research policy issues on the
7. Academic Federation (AF) Committee on
Research, 1996-1997; 1997-1998; 1998-1999; Chair of Committee 1997-1998
and 1998-1999. Committee dealt with campus-wide research issues such as funding
and research policies. As Chair oversaw the annual call,
review, and disbursement of AF Research Travel Awards. Established AF Excellence in Research Award. Established methods for determining AF PI extramural funding.
8. Joint
Campus Committee on Information Technology (JCCIT), 1996-1997;
1997-1998. Member of sub-committee that authored recommendations that led to
formation of the present Academic Computing Coordinating Council. Provided recommendation to campus IT division on issues such as
support for instruction, technology training, support for research, modem pool,
classroom Net connectivity.
Search and Ad hoc Committees
1. Vice-Chancellor for Research search
committee, 2001-2002.
2. Associate Vice-Chancellor for Research
search committee, 2003.
3. Vice-Chancellor for Research Intramural
Grants Review Committee, 2003. Made recommendations for
revision of procedures governing intramural funding on the
Publications
1. 1979 G.N. La Mar and J.S.
de Ropp. Assignment of Exchangeable
Histidine Resonances in High Spin Ferric Heme Proteins. Biochem. Biophys Res.
Comm. 90, 36-41.
2. 1979 K.M. Smith, K.C. Langry, J.S. de Ropp. Electrophilic Deuteration of Natural Porphyrin Derivatives. J. Chem Soc. Chem.
Comm. 1001-1003.
3. 1980 G.N. La Mar and J.S.
de Ropp. Proton NMR
Characterization of the Electronic Structure of HRP-I. J. Am. Chem. Soc. 102, 395-397.
4. 1980 G.N. La Mar, J.S. de
Ropp, K.M. Smith, K.C. Langry. Proton
NMR Study of the Heme Crevice in Horseradish Peroxidase.
J. Biol. Chem. 255, 6646-6652.
5. 1980 G.N. La Mar, J.S. de
Ropp, K.M. Smith, K.C. Langry. NMR
Characterization of Heme Rotational Disorder. J. Am. Chem. Soc. 102,
4833-4835.
6. 1981 G.N. La Mar, J.S. de
Ropp, K.M. Smith, K.C. Langry. Proton
NMR Investigation of Compound I of Horseradish Peroxidase.
J. Biol. Chem. 256, 237-243.
7. 1982 G.N. La Mar, K.M.
Smith, J.S. de Ropp, P.D. Burns, K.C. Langry. Heme Orientational Heterogeneity
in Hemoproteins as Detected by Proton NMR In: Symposium on Interaction Between
Iron and Proteins in Oxygen and Electron Transport, C. Ho, Ed.
8. 1982 G.N. La Mar and J.S.
de Ropp. Proton NMR
Characterization of the State of the Axial Imidazole in Reduced Horseradish Peroxidase. J.
Am. Chem. Soc. 104, 5203-5207.
9. 1982 G.N. La Mar, J.S. de
Ropp, V.P. Chacko, J.D. Satterlee,
J.E. Erman. Axial Histidyl Imidazole
Non-Exchangeable Proton Resonances as Indicators of Imidazole Hydrogen Bonding
in Ferric Cyanide Complexes of Heme Peroxidases.
Biochem. Biophys. Acta 708, 317-325.
10. 1982 G.N. La Mar, V.P. Chacko, J.S. de Ropp. The State
of
11. 1983 G.N. La Mar, J.S.
de Ropp, L. Latos-Grazynski, A.L. Balch,
R.D. Johnson, K.M. Smith, D.W. Parrish, R.J. Cheng. Proton
NMR Characterization of the Ferryl Group in Model Heme Complexes and Heme
Proteins. J.Am.Chem. Soc. 105, 4576-4580.
12. 1983 G.N. La Mar, J.S.
de Ropp, K.M. Smith, K.C. Langry. Determination by 1H
NMR of the Orientation of Modified Hemes Incorporated into Horseradish Peroxidase. J. Am.
Chem. Soc. 105, 782-787.
13. 1984 J.S. de Ropp and F.A. Troy. Chemical Synthesis and 2H NMR Investigations of Polyisoprenols:
Dynamics in Model Membranes. Biochemistry
23, 2691-2695.
14. 1984 J.S. de Ropp, G.N. La
Mar, K.M. Smith, K.C. Langry. NMR
Investigations of Low Spin Ferric Horseradish Peroxidase Complexes. J. Am. Chem. Soc. 106, 4438-4444.
15. 1985 J.S. de Ropp and F.A. Troy 2H NMR Investigation of the
Organization and Dynamics of Polyisoprenols in Membranes.
J. Biol. Chem. 260,
15669-15674.
16. 1985 J.S. de Ropp, V. Thanabal, G.N. La Mar. NMR Evidence for a
17. 1986 V. Thanabal, J.S. de Ropp, G.N. La Mar. Determination of Vinyl
Orientation in Resting State and Compound I of Horseradish Peroxidase by the 1H NMR Nuclear Overhauser Effect. J.
Am. Chem. Soc. 108, 4244-5.
18. 1987 J.D. Satterlee, J.E. Erman, J.S. de Ropp. Proton Hyperfine Resonance Assignments in Cyanide
Ligated Cytochrome c Peroxidase Using
the Nuclear Overhauser Effect. J. Biol.
Chem. 262, 11578-11583.
19. 1987 V. Thanabal, J.S. de Ropp, G.N. La
Mar 1H NMR Study of the Electronic and Molecular
Structure of the Heme Cavity in Horseradish Peroxidase: Complete Heme Resonance
Assignments Based on Saturation Transfer and Nuclear Overhauser Effects. J. Am. Chem. Soc. 109, 265-272.
20. 1987 J.S. de Ropp, M.J.
Knudsen, F.A. Troy. 2H NMR Investigation of the
Dynamics and Conformation of Polyisoprenols in Model
Membranes.
Chemica Scripta 27, 101-108.
21. 1987 V. Thanabal, J.S. de Ropp, La Mar G.N..
Identification of the Catalytically Important Amino Acid
Residues in Ferric Low Spin Horseradish Peroxidase Using Nuclear Overhauser
Effect Measurements. J. Am. Chem.
Soc. 109, 7516-7525.
22. 1988 V. Thanabal, J.S. de Ropp, G.N. La Mar. Proton NMR
Characterization of the Catalytically Relevant Proximal and Distal
Hydrogen-Bonding Networks in Ligated Resting State Horseradish Peroxidase. J. Am.
Chem. Soc..
110, 3027-3035.
23. 1988 J.H. Theis, J.S. de Ropp, R.G. Schwab, J. Banks, K. Levine. Nuclear Magnetic Resonance to Differentiate Bear, Pig and Cow Bile
for Forensic Investigations. Wildl. Soc. Bull. 16, 430-433.
24. 1988 G.N. La Mar, M.J.
Chatfield, D.H. Peyton, J.S. de Ropp, W.S. Smith, R. Krishnamoorthi,
J.D. Satterlee, J.E. Erman.
Solvent Isotope Effects on NMR Spectral Parameters in
High-Spin Ferric Hemoproteins. Biochim. Biophys. Acta 956, 267-276.
25. 1988 V. Thanabal, G.N. La Mar, J.S. de Ropp
A Nuclear Overhauser Effect
Study of the Heme Crevice in
26. 1989 M.N. Goodman, L.K. Masuoka, R. Dietrich, P. Luu,
J.S. de Ropp, A.D. Jones,. Increased Formation of Alanine by Rat Skeletal Muscle During the Fasted-Refed Transition: Role in Hepatic Glycogen Repletion by the
Indirect Pathway. Biochem. Biophys. Res. Comm. 159, 522-527.
27. 1989 J. Reed, J.S. de
Ropp, J. Trewhella, D.B. Glass, W.K. Liddle, E.M. Bradbury, V. Kinzel,
D.A. Walsh. Conformational Analysis of PKI(5-22)Amide,
The Active Inhibitory Fragment of the Inhibitor Protein of the Cyclic
AMP-Dependent Protein Kinase. Biochem. J. 264, 371-380.
28. 1990 R.E. Arasasingham, A.L. Balch, C.R. Cornman, J.S. de Ropp, K. Eguchi,
G.N. La Mar. 1H NMR Studies of Iron(III) Porphyrins With Axial Phenoxide
or Thiophenoxide Ligands. Inorg. Chem. 29, 1847-1850.
29. 1991 J.S. de Ropp, L.P.
Yu, G.N. La Mar. 2D NMR of Paramagnetic Metalloenzymes: Cyanide Inhibited Horseradish Peroxidase. J. Biomol. NMR 1,
175-190.
30. 1991 J.S. de Ropp and G.N. La Mar. 2D NMR Assignment of Hyperfine
Shifted Resonances in Strongly Paramagnetic Metalloproteins:
Resting State Horseradish Peroxidase. J. Am. Chem. Soc. 113, 4348-4350.
31. 1991 K.A. Keating, J.S.
de Ropp, G.N. La Mar, A.L. Balch, F.Y. Shiau, K.M. Smith. Assignment by 2D NMR
Bond Correlation Spectroscopy of Hyperfine-Shifted and Strongly Relaxed Protons
in Iron Porphyrin and Chlorin Complexes. Inorg. Chem. 30, 3258-3263.
32. 1991 J.S. de Ropp, G.N.
La Mar, H. Wariishi, M.H. Gold. NMR Study of the
Active Site of Resting State and Cyanide-inhibited Lignin Peroxidase from Phanerochaete chrysosporium. J. Biol. Chem. 266, 5001-5008.
33. 1992 G.N. La Mar, G.
Hernandez, J.S. de Ropp. 1H NMR Investigation of the Influence of Interacting Sites on the
Dynamics and Thermodynamics of Substrate and Ligand Binding to Horseradish Peroxidase. Biochemistry
31, 9158-9168.
34. 1993 M. Sette, J.S. de Ropp, G. Hernandez, G.N. La Mar.
Determination of Interproton Distances From NOESY Spectra in the Active Site of Paramagnetic Metalloenzymes: Cyanide Inhibited Horseradish Peroxidase. J. Am.
Chem. Soc. 115, 5237-5245.
35. 1993 G.N. La Mar and
J.S. de Ropp. NMR Methodology For
Paramagnetic Proteins. in Biological
Magnetic Resonance (Berliner, L.J. and Reuben, J., eds.) Plenum Press,
36. 1993 J.S. de Ropp and G.N. La Mar. Horseradish Peroxidase
Heme Pocket Dynamics: NMR Study of Exchangeable
Protons. in Plant Peroxidases:
Biochemistry and Physiology (Welinder, K.G.,
Rasmussen, S.K., Penel, C. and Greppin,
H., eds)
37. 1994 Z. Chen, J.S. de
Ropp, G. Hernandez, G.N. La Mar. 2D NMR Approaches to Characterizing the
Molecular Structure and Dynamic Stability of the Active Site for
Cyanide-Inhibited Horseradish Peroxidase. J. Am. Chem. Soc. 116, 8772-8783.
38. 1995 G.N. La Mar, Z.
Chen, J.S. de Ropp. Assignment Strategies and
Structure Determination in Cyanide-Inhibited Heme Peroxidases
in Nuclear Magnetic Resonance of
Paramagnetic Macromolecules (G.N. La Mar ed) Kluwer
Academic Publishers, Dordrecht, pp. 55-72.
39. 1995 J.S. de Ropp, Z.
Chen, G.N. La Mar. Identification of Residues in the Aromatic Substrate Binding
Site of Horseradish Peroxidase by 1H NMR Studies on Isozymes. Biochemistry
34, 13477-13484.
40. 1997 J.S. de Ropp, P. Mandal, S.L. Brauer, G.N. La Mar.
Solution NMR Study of the Electronic and Molecular Structure of the Heme Cavity
in High-Spin, Resting State Horseradish Peroxidase. J. Am. Chem. Soc. 119, 4732-4739.
41. 1997 W.L. Kerr, C.J.
Clark, M.J. McCarthy, J.S. de Ropp. Freezing Effects
in Fruit Tissue of Kiwifruit Observed by Magnetic Resonance Imaging. Scientia Horticulturae, 69,
169-179.
42. 1998 J.S. de Ropp, J.H. Theis, J.I. Mead, V.C. Bleich. Limitations of Nuclear Magnetic Resonance Analysis of Fecal Bile
for Taxonomic Identification of Contemporary and Extinct Mammals.
43. 1999 J.S. de Ropp, P. Mandal, G.N. La Mar. Solution 1H NMR Investigation of the Heme Cavity and Substrate Binding Site in
Cyanide-Inhibited Horseradish Peroxidase. Biochemistry, 38, 1077-1086.
44. 1999 U. Kolczak, J.B. Hauksson, N.L.
Davis, U. Pande, J.S. de Ropp, K.C. Langry, K.M. Smith, G.N. La Mar. 1H NMR
Investigation of the Role of Intrinsic Heme versus Protein-Induced Rhombic
Perturbations on the Electronic Structure of Low-Spin Ferrihemoproteins:
Effect of Heme Substituents on Heme Orientation in Myoglobin.
J. Am. Chem. Soc. 121, 835-843.
45. 1999 G.N.
La Mar, J.D. Satterlee, J.S. de Ropp.
Nuclear Magnetic Resonance of
46. 2000 D.-M.
Jung, J.S. de Ropp, S.E. Ebeler. Study
of Interactions between Food Phenolics and Aromatic
Flavors Using One- and Two-Dimensional NMR Spectroscopy. J. Agric. Food Chem. 48, 407-412.
47. 2000 J.S.
de Ropp and M.J. McCarthy. NMR Analysis in the
Analysis of Foodstuffs and Plant Materials, IN: Encyclopedia of Analytical Chemistry: Instrumentation and Applications
(Meyers, R.A. ed.) John Wiley & Sons,
48. 2001 A. Asokan, J.S. de Ropp, S.L. Newmyer, P.R. Ortiz de Montellano, G.N. La Mar. Solution 1H NMR of the Molecular and Electronic Structure of
the Heme Cavity and Substrate Binding Pocket of High-Spin Ferric Horseradish
Peroxidase: Effect of His42Ala Mutation. J. Am. Chem.
Soc. 123, 4243-4254.
49. 2002 K. Yamada, E.R. Wisner, J.S. de Ropp,
R.A. LeCouteur, L.D. Tripp. Technical
Parameters Affecting Image Characteristics in In
Vivo MR Microscopy of the Mouse. Veterinary
Radiology & Ultrasound. 43,
518-527.
50. 2002 J.S. de Ropp, S. Sham, A Asokan,
S. Newmyer, P.R. Ortiz de Montellano,
G.N. La Mar.. Influence of the Distal His in Imparting
Imidazolate Character to the Proximal His in Heme Peroxidase: 1H NMR
Spectroscopic Study of Cyanide-Inhibited His42Ala Horseradish Peroxidase. J. Am.
Chem. Soc. 124, 11029-11037.
51. 2002 D.M. Jung, J.S. de Ropp, S.E. Ebeler. Application of pulsed field gradient NMR techniques
for investigating binding of flavor compounds to macromolecules. J. Agr. & Food Chem. 50,
4262-4269.
52. 2002 J.B. MacMillan,
M.A. Ernst-Russell, J.S. de Ropp, T.F. Molinski. Lobocyclamides A-C, Lipopeptides from a Cryptic Cyanobacterial
Mat Containing Lyngbya confervoides.
J. Org. Chem. 67, 8210-8215.
53. 2003 J.H. Walton, J.S. de Ropp, M.V. Shutov, A. Goloshevsky, M.J.
McCarthy, R.L. Smith, S.D. Collins. A Micromachined
Double Tuned NMR Microprobe. Anal. Chem. ,
75, 5030-5036.
FUNDING
Ongoing
Title: Development of a Micro NMR System
Agency:
Role: Jeffery S. de Ropp, co-PI
10/01/01-09/30/04; $193,205 TDC (~$64,400/year,
renewed each year)
Goals: develop micro NMR probes
Completed
Title: Upgrade of a Shared Campus NMR Facility
Agency: National Science Foundation; grant OSTI 9724412
Principal Investigator: G. Smith (J. de Ropp,
co-PI)
Goals: Equipment
grant, funded complete upgrades of 500 MHz spectrometer and 7T scanner
Title: Purchase of a High-Field Cryoprobe
Agency: National Science Foundation
Principal Investigator: G. Smith (J. de Ropp,
co-PI)
Goals: Equipment
grant; funded purchase of cryoprobe
for 600 MHz spectrometer
Title: Purchase of a High Field NMR Spectrometer
Agency: National Institutes of Health; grant RR11973
Principal Investigator: G. La Mar (J. de Ropp,
co-investigator)
Goals: Equipment
grant; funded purchase of 600 MHz spectrometer
Major role in six additional
equipment grants to NIH/NSF that produced $1,991,000 TDC.